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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Mol Immunol. 2007 Dec 21;45(7):1825–1836. doi: 10.1016/j.molimm.2007.11.002

Figure 1.

Figure 1

Primary B lymphocytes and the A20 B cell line express Lsc as a large molecular weight homo-oligomer. A) Western analysis of Lsc expression in primary CD19+ B lineage bone marrow cells (lane 1) and CD19+ (lane 2) and CD19−(lane 3) splenocytes as revealed with rabbit anti-mouse Lsc antisera. B) Whole cell lysates from the A20 cell line (top) or purified splenic B lymphocytes (bottom) were size fractionated as described for Figure 1 and Lsc content revealed using anti-Lsc antisera. C) Whole cell lysates from A20 and primary B lymphocytes were immunoprecipitated with either pre-immune rabbit sera (rabbit Ig) or anti-Lsc antisera (anti-Lsc) in the same buffer as that used for size fractionation, resolved by SDS/PAGE, and immunoprecipitated proteins stained with Coomassie Blue.