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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Mol Immunol. 2007 Dec 21;45(7):1825–1836. doi: 10.1016/j.molimm.2007.11.002

Figure 2.

Figure 2

A carboxy-terminal Lsc protein interferes with the assembly of large molecular weight Lsc oligomers. A) Transgene expression in A20 transfectants as determined by Western analysis of transfectants expressing either YFP (lane 1), Lsc-FLAG (lane 2), CFP (lane 3) or LscCT-CFP (lane 4). Blots were revealed with anti-LscCT antisera against the carboxy-terminal Lsc domain. Size exclusion chromatography and SDS/PAGE of A20 transfectants reveal B) Lsc-FLAG is predominantly associated with complexes displaying 500 kDa molecular weight as determined by Western blotting with anti-FLAG antibody and C) LscCT associates with endogenous Lsc in distinct molecular weight complexes as determined using anti-Lsc antisera. Putative oligomeric complexes are shown with predicted and experimentally determined molecular weights.