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. 2008 Apr 21;181(2):241–254. doi: 10.1083/jcb.200710019

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Copyright © 2008, The Rockefeller University Press

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Figure 4. — Aurora B activity is required for kinetochore maintenance in cycled extracts. (A and B) Cycled X. laevis extracts containing replicated chromatids and assembled kinetochores were tested for their sensitivity to hesperadin (or DMSO). After 15 min in hesperadin, Knl1 (A) and Mis12 (B) were displaced from kinetochores. (C) Maintenance reactions were repeated in CSF and cycled egg extracts. Aurora B complex proteins were displaced from chromatin by the addition of hesperadin after assembly. X. laevis S3 cells also lost Aurora B staining on chromatin after treatment with hesperadin. Bars, 5 μm.