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. 2008 Apr 21;181(2):293–307. doi: 10.1083/jcb.200710215

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Copyright © 2008, The Rockefeller University Press

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Figure 5. — eIF4GI depletion blocks Skp2 mRNA translation. (A) Immunoblot of the indicated proteins in cells transfected with specific siRNAs. (B) Control or eIF4GI-depleted cells treated with proteasome inhibitor and proteins detected by immunoblot. (C) Total Skp2 mRNA levels in eIF4GI-silenced cells, determined by qRT-PCR analysis. (D) qRT-PCR analysis of Skp2 and Hsp27 mRNA in polysomes with eIF4GI depletion. (E) Immunoprecipitation of ³⁵S-Met labeled lysates with the indicated antibodies. Error bars represent standard deviation, calculated from at least three independent experiments. Statistical analysis used the t test; *, P < 0.05.