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. 2008 Apr 21;181(2):309–320. doi: 10.1083/jcb.200712068

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Copyright © 2008, The Rockefeller University Press

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Figure 3. — HA glycans associated with calnexin are preferentially reglucosylated by GT1. (A) HA was translated in the presence of wild-type or MI8-5 SP cells treated with DMJ in the presence or absence of DNJ at 27°C for 60 min. After lysis of the SP cells, radiolabeled HA was immunoprecipitated with polyclonal HA, calnexin (αCNX), or calreticulin (αCRT) antisera. Samples were resolved via 7.5% SDS-PAGE. (B) Quantifications of A (n = 3). Error bars show SD.