Figure 6. Effect of indirect exposure of the pseudomonad strains and cyanide on the transcription of the epsA operon in B. subtilis.

Strain Marburg thrC::epsA-lacZ (NRS1663) was grown in biofilm medium under biofilm formation conditions at 37°C with or without exposure to pseudomonad strains and HCN. Growth (A) and β-galactosidase activity (B) were measured at regular intervals and plotted as a function of time. Data is the mean±SD of 12 replicates and the experiment was repeated on two independent occasions. These experiments were repeated on at least 3 independent occasions and a representative plot is shown.