Figure 2.

Comparisons of Fas expression levels and sensitivity to Fas-mediated apoptosis between CD5⁺ and CD5⁻ B cells. Splenic CD5⁺ and CD5⁻ B cells from 2-month-old NZB/W F₁ mice were FACS-sorted and stimulated with anti-CD40 mAb for 3 days, followed by incubation in the presence (Anti-Fas-treated) or absence (Non-treated) of anti-Fas mAb for 24 hr. Fas expression levels on PI-negative living cells were examined by three-color flow cytometry. Dotted line depicts autofluorescence.