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Effect of botulinum toxin E expression on stimulation-dependent vesicle acidification
Stimulation-dependent acidification of intracellular sites in native Neuro2A cells (○) and cells expressing BoNT/E-LC (•), as observed by acridine orange accumulation after exposure to 50 mm K+ (added at time = 0 min). Each data point represents n = 3 experiments (± s.e.m.), with >30 cells imaged per experiment.
