Figure 7.

Effects of the nicotinic receptor agonist DMPP on the acidification and morphology of vesicles of chromaffin cells in vivo

A, vehicle-injected control. Both active and stable dense-cored vesicles are present. Although few are labelled with DAMP, a highly labelled lysosome (L) is present. The trapping of DAMP within the lysosome indicates that the injected DAMP has entered the chromaffin cell and that its distribution reflects the ΔH⁺ gradient across the membrane of this organelle. B, DMPP treated. More chromaffin vesicles exhibit an active morphology (arrows) with halo around the dense cores. Note also that many of the vesicles are now labelled with DAMP (see also Table 3). Scale bars: 200 nm.