Figure 8.

Effects of stimulation on the acidification of small and large synaptic vesicles of rat myenteric neurons

A, in control preparations of rat myenteric neurons in which spontaneous activity of neurons was blocked by the addition of tetrodotoxin, synaptic vesicles contained very little DAMP. In contrast, lysosomes, which are abundant in the enteric perikarya, were highly labelled (inset). B, stimulation of myenteric neurons with Ca²⁺ receptor agonist, Gd³⁺ (500 μm for 30 min) in the presence of DAMP. Arrows indicate small and large synaptic vesicles labelled with DAMP. C, rat myenteric neurons were stimulated with the Na⁺ channel activator veratridine (1 μm for 30 min) in the presence of DAMP. Both small clear and large dense-cored synaptic vesicles were labelled (arrows). Scale bars: 100 nm.