Figure 2.

TLC analysis of partially purified root extracts from wild-type and mutant oats. The fluorescent avenacins, A-1 and B-1, were visualized under UV illumination (Upper), and all four saponins were detected using a chromogenic reagent (Lower). Preparations enriched for each of the four avenacins (3 μg per lane) are on the Right, and extract from wild-type (WT) oat roots is on the Left. The major fluorescent compound present in the wild-type root extract is avenacin A-1, and the minor one with a higher R_f value is avenacin B-1.