Figure 2.

Two-hybrid assay for protein–protein interactions among Arabidopsis flavonoid enzymes. Growth of transformed HF7C yeast cells was assayed on his-, 5 mM 3-aminotriazole medium at 30°C for 4 days. The top row illustrates a representative interaction assay. The Gal4_1–147 DNA-binding domain (DB) fusion is indicated at the top of each plate, and the Gal4_768–881 transactivation domain (TA) fusion is indicated in each sector. The bottom row shows the controls—yeast cells transformed with single plasmids carrying TA fusions, as indicated.