Figure 4.

Coimmunoprecipitation of flavonoid enzymes. Affinity-purified anti-CHI bound to Affi-Gel Hz resin was used to precipitate CHI from crude soluble protein extracts of 3-day-old seedlings. A control experiment was performed by using the corresponding preimmune serum. Immunoblot analysis was used to identify other flavonoid enzymes that coimmunoprecipitated with CHI. T, total lysate; U, unbound; and P, precipitated proteins. Asterisks indicate the positions of the light and heavy chains of the IgY (anti-CHI) or IgG (preimmune serum) that were eluted from the resin together with the immunoprecipitated proteins.