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. 1999 Oct 26;96(22):12929–12934. doi: 10.1073/pnas.96.22.12929

Figure 5.

Figure 5

Analysis of the two molecular mass variants of CHI. Samples of plant extracts treated with specific compounds were fractionated by using reducing SDS/PAGE and then assayed for CHI by immunoblot analysis. Time points were taken at 1, 4, or 7 h for plant lysates with and without 2 mM 2-mercaptoethanol (+/− me). Samples were collected after 30 min of incubation with 1 M Tris, pH 7.2 (T); 0.2 M KOH (OH); 1 M hydroxylamine, pH 7.2 (H); or 5 M 2-mercaptoethanol (me). S, plant lysate, used as a size standard, that contains both molecular mass species of CHI after incubation in buffer containing 2-mercaptoethanol for 4 h. The two bands migrate at approximately 31 and 26 kDa relative to molecular mass standards.