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Activity of the recombinant isoform II of protoporphyrinogen IX oxidase. An E. coli clone expressing the mitochondrial protoporphyrinogen IX oxidase (PPX II) was lysed. Enzyme activity of the bacterial homogenate (1) in the presence of 100 nM (2), 1 μM (3), and 10 μM (4) acifluorfen, and endogenous protoporphyrinogen IX oxidase activity of the E. coli strain SG 13009 harboring the plasmid pDS 56 (5) were measured as described in Material and Methods.
