Table 1.

Transient gene expression in transgenic Msenod12A–uidA roots

	Plants, no.	Roots, no.	% + roots	No. + per plant	No. + per root	No. + per + root
Optimization bombardments
35S–uidA	10	37	97	20	5.5  ± 0.6	5.7  ± 0.6
35S vector	16	72	0	0	0  ± 0	0  ± 0
Au	10	30	0	0	0  ± 0	0  ± 0
enod40 bombardments
35S–Mtenod40	28	148	15	1.0	0.19  ± 0.04	1.3  ± 0.1
35S–Mtenod40–Δ7	18	86	10	0.60	0.13  ± 0.04	1.1  ± 0.1
35S–Mtenod40–Δ5	25	105	14	1.2	0.30  ± 0.1	2.1  ± 0.6
35S–Mtenod40 (+N)	10	21	0	0	0  ± 0	0  ± 0
Control DNA	40	142	0	0	0  ± 0	0  ± 0
NF + Au	6	38	24	2.7	0.42  ± 0.1	1.8  ± 0.3
BAP + Au	6	48	17	2.5	0.31  ± 0.1	1.9  ± 0.2
BAP − Au	4	15	13	0.50	0.13  ± 0.09	1.00  ± 0

Number of positives (+, see below) were counted after particle gun bombardment of roots. Optimization bombardments: positives correspond to nondividing cells transiently expressing the introduced 35S–uidA gene. enod40 bombardments: positives correspond to sites showing cortical cell divisions (ccd) and expression of the endogenous Msenod12A–uidA gene. Numbers of positives are given (mean ± SEM). % + roots is the percentage of roots showing positives per total number of roots. No. + per + root is the number of positives per root showing a response. Au, bombardment with gold particles without bound DNA. +N, cuttings were grown in the presence of combined nitrogen. Control DNA, 35S vector or vector expressing an irrelevant gene. NF, 0.1 μM NodRm-IV (C16:2, AcS) Nod factor (kindly provided by M. Schultze) was added to the roots before bombardment (Au). BAP, 10 μM benzylaminopurine was similarly added, with (+Au) or without (−Au) a following bombardment. Nod factor and BAP treatments induce ccd and Msenod12A (4).