Table 2.
The latency for the appearance of a tumor after DMBA exposure, number of tumors per animal (tumor multiplicity), mean area of tumors at first detection, and tumor growth rate in DMBA-treated rats that were exposed to low-fat or high-fat diets in utero or to oil vehicle or E2 daily during gestation days 14-20
| Treatment | Tumor latency, weeks | n | Tumor multiplicity, no. tumors/rat | n | Tumor area, mm2 | n | Tumor doubling time, days | n |
|---|---|---|---|---|---|---|---|---|
| Diet in utero | ||||||||
| Low fat | 14.2 ± 0.6 | 17 | 1.2 ± 0.1 | 17 | 81.3 ± 11.4 | 17 | 8.4 ± 3.7 | 11 |
| High fat | 11.4 ± 0.5* | 36 | 1.4 ± 0.1 | 36 | 60.0 ± 12.6 | 36 | 13.8 ± 2.2 | 19 |
| E2, gestation days 14–20 | ||||||||
| Vehicle only | 14.2 ± 1.7 | 5 | 1.0 ± 0.0 | 5 | 122.0 ± 80.0 | 5 | 30.8 ± 12.9 | 3 |
| E2 | 12.7 ± 0.7 | 13 | 1.2 ± 0.2 | 13 | 67.0 ± 30.0 | 13 | 21.0 ± 3.8 | 10 |
n = number of tumors. Low-fat diet, n = 60, 12% calories from fat. High-fat diet, n = 60, 46% calories from fat. Oil vehicle, n = 26. E2, 20 ng, n = 25, daily during gestation days 14–20.
*
P < 0.002.