Table 2.

SHIV-HXBc2 was not isolated from PBMC of env DNA/env DNA plus o-gp160 vaccinated rhesus monkeys after virus challenge

Prime/boost regimen	Monkey	SHIV isolation week after challenge
		2	3	4	6	8	11	13	17	21
env DNA/env	0092	−	−	−	−	−	−	−	−	−
DNA plus o-gp160	0003	−	−	−	−	−	−	−	−	−
naive/o-gp160	0094	+	+	+	+	+	+	+	+	+
	0076	+	+	+	+	+	+	+	+	+
	0342	+	+	+	+	+	+	+	+	+
naive/vector	0053	+	+	+	+	+	+	+	+	+
DNA plus OVA	0065	+	+	+	+	+	+	+	+	+
	0059	+	+	+	+	+	+	+	+	+

PBMCs of the rhesus monkeys were obtained at the noted times after SHIV-HXBc2 challenge, stimulated with Con A for 18 h, then depleted of CD8+ cells using immunomagnetic beads and maintained in culture for 2 weeks in IL-2-containing medium. The medium was changed regularly, and supernatants were assessed for SIVp27 antigen as determined by enzyme immunoassay. A virus isolation is noted as + if the supernatants of that culture contained measurable SIVp27 antigen.