Figure 2.

Effects of salt and temperature on bypass transcription. (A) Transcription reactions were done as in Fig. 1, lane 2, but at different temperatures. The amount of RNA produced at each temperature was normalized to activated transcription as in Fig. 1, lane 1. (B) Transcription reactions were done as in Fig. 1, lane 3. To increase the ionic strength of the reaction, KCl was added to supplement the approximately 40 mM NaCl contributed by various components of the reaction. Transcription is normalized to activated wild type and is plotted against total K⁺ and Na⁺ ion.