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Domain closure. The Cα trace of the loop region and side chains of relevant residues are shown. (Upper Left) Unligated P. aeruginosa catabolic OTCase structure, with the active site open to solvent (13). (Lower Left) E. coli OTCase/PALO structure, with the two domains closed. (Right) The simulated annealing omit Fo − Fc map (contour level 2.0 σ, residues 233–239, and PALO omitted from minimization and phase calculation), confirms the positions of these residues and the ligand bound.
