Figure 1.

Stopped flow traces of the reduction of A95G and wild-type lactate oxidase with l-mandelate. Equal volumes of argon-equilibrated enzyme solution (12 μM final concentration) were mixed with argon-equilibrated l-mandelate solutions of different concentration, and reduction of the flavin monitored at 456 nm. Conditions were pH 7.0 at 25°C. The traces with wild-type enzyme, curves from right to left, were obtained with l-mandelate concentrations of 1, 1.5, 2.5, 5, 10, and 25 mM. The traces with A95G enzyme, curves right to left, were obtained with l-mandelate concentrations of 1, 2, 5, and 10 mM.