. 1997 Sep 2;94(18):9590–9595. doi: 10.1073/pnas.94.18.9590

Table 1.

Reduction rate constants (k_red) and dissociation constants (K_d) for reaction of lactate oxidases with α-¹H- and α-²H-para-substituted mandelates at 25°C and pH 7

Mandelate derivative	Wild-type lactate oxidase					A95G lactate oxidase
	k_red, sec⁻¹		K_d, mM		R-value	k_red, sec⁻¹		K_d, mM		R-value
	α-¹H-	(α-²H-)	α-¹H-	(α-²H-)	R-value	α-¹H-	(α-²H-)	α-¹H-	(α-²H-)	R-value
DL- OH-	0.009		2.03		0.9856	0.29		16.2		0.9996
		(ND)		(ND)			(ND)		(ND)
L- CH₃O-	0.055		0.83		0.9939	1.61		4.0		0.9993
		(0.009)		(0.98)	0.9854		(0.38)		(5.8)	0.9998
L- CH₃-	0.048		0.34		0.9721	3.17		3.9		0.9998
		(0.007)		(0.34)	0.8226		(0.60)		(3.5)	0.9994
L- H-	0.038		0.64		0.9747	5.88		6.3		0.9999
DL- H-		(0.010)		(0.61)	0.9888		(1.52)		(4.3)	0.9995
L- F-	0.037		0.36		0.9558	1.85		3.6		0.9998
		(ND)		(ND)			(ND)		(ND)
L- Cl-	0.023		0.23		0.8972	0.98		1.3		0.9962
		(0.003)		(0.12)	0.5882		(0.18)		(1.8)	0.9978
L- CF₃-	0.018		0.51		0.9064	0.20		3.1		0.9943
		(0.003)		(0.37)	0.9883		(0.05)		(5.6)	0.9987
L- NO₂-	0.017		0.34		0.9785	0.21		3.2		0.9977
		(ND)		(ND)			(ND)		(ND)

ND, no data.