Figure 5.

Effect of PLC_β inhibition on the SP-induced [Ca²⁺]_i change. Cells expressing the full-length receptor (—) or the truncated receptor (- - -) were preincubated with (A) U73122 (10 μM), or (B) U73343 (10 μM). In calcium-free HBS buffer (containing 1 mM EGTA), changes in [Ca²⁺]_i were measured upon application of SP (1 nM), followed by addition of Ca²⁺ (2 mM) to the buffer. The traces are from representative experiments repeated at least three times.