Skip to main content
. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Biol Reprod. 2008 Jan 9;78(4):667–672. doi: 10.1095/biolreprod.107.066019

Figure 2.

Figure 2

GPR3 RNA is expressed in human oocytes. RT-PCR using cDNA derived from 6.5 human oocyte equivalents amplified bands for GPR3, while GPR12 was not detected. All of the RT-PCRs shown above were performed on the same cDNA and run on the same gel. The presence of GPR3, as well as actin, demonstrate the quality of the cDNA. Possible genomic contamination was ruled out by performing PCR on the same batch of RNA that had not undergone reverse transcription. We estimate the amount of cDNA used for these PCR reactions corresponded to ∼20 ng total RNA. This figure is based on the amount of RNA in a mouse oocyte (∼1 ng; [3]), and taking into account that human oocytes contain 3X the volume of mouse oocytes. Each experiment was performed 7 times or more, using at least 6 different batches of cDNA.