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Site-specific forward (pyk-F) and reverse (pyk-R) primer sequences used for verification of deletion of the pyk gene encoding pyruvate kinase in C. glutamicum by PCR.
| Primer direction | Primer sequence |
| Forward | pyk-F: 5'-GATCC TCGAG CTCTA CTGAG CTGGT TTAT -3' |
| Reverse | pyk-R: 5'-GATCG GATCC CGGTC AACAT AGAGC TCA -3' |
