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. 2008 Mar 17;3:7. doi: 10.1186/1749-8104-3-7

Figure 4.

Figure 4

AX-1 expression in postmitotic granule cells precedes expression of NgCAM, NrCAM, and Contactin/F11.(a,d,g,j,m) AX-1 is a marker for postmitotic granule cells that start to extend processes in the inner EGL [6,8]. (a-c) Double-staining of sagittal sections taken from HH35 brains demonstrated that AX-1 (a) labeled young postmitotic granule cells in the inner EGL (iEGL) that were not yet NgCAM-positive (b; merged in c). Therefore, an overlap between AX-1 and NgCAM was only found in the ML, where older granule cells are located (c). (d-f) Double staining for AX-1 (d) and NrCAM (e) revealed that there was less overlap between AX-1 and NrCAM (f) compared to AX-1 and NgCAM (c). (g-i) Similarly, there was little overlap (i) between AX-1 (g) and Contactin/F11 (h), consistent with previous reports [39]. (j-l) AX-1 is expressed in postmitotic granule cells but not in granule cell precursors in the outer EGL (oEGL) that are still proliferating (j). Precursors and postmitotic granule cells are identified by Pax6 staining (k) [42]. The overlap between AX-1 and Pax6 is restricted to the inner EGL (l). (m-o) AX-1 reactivity (m) mostly overlapped with RMO270 reactivity (n), demonstrating that the vast majority of the neurofilament signal at HH35 was generated by granule cells (o). Bar: 50 μm.