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Parameter settings relaxed for round three in the primer design process.
| Primer length, round 3 | 16 – 28 nucleotides |
| Product length, round 3 | 80 – 200 nucleotides |
| Max. primer melting temperature diff., round 3 | 4.0 ° |
| Max. base repeat, round 3 | 4 (e.g., GGGG in a primer is allowed, although not in the 3'-end) |
