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. 2008 Apr 22;118(5):1645–1656. doi: 10.1172/JCI32691

Figure 7. Regulation of oxidant stress and Nox4 by adiponectin.

Figure 7

(A) Urinary levels of hydrogen peroxide were reduced by gAd, fAd, or AICAR treatment in Ad–/– mice (n = 7–10 per group). *P < 0.05 versus WT; #P < 0.05 versus Ad–/–. (B) Glomerular 8-OHdG was increased in Ad–/– kidneys and reduced with gAd, demonstrated by light microscopy immunostain and quantitation of 8-OHdG–positive cells per glomerulus (n = 4 per group). *P < 0.05 versus WT; **P < 0.05 versus Ad–/–. (C) Glomerular nitrotyrosine was increased in Ad–/– kidneys and reduced with gAd treatment, demonstrated by light microscopy immunostain and quantitation of nitrotyrosine staining per glomerulus (n = 4 per group). *P < 0.05 versus WT; **P < 0.05 versus Ad–/–. (D) Nox4 was present in podocytes, as well as other glomerular cells and tubular cells, as demonstrated by double labeling with synaptopodin in WT kidney. Insets show representative background staining without primary antibody. (E) Light microscopy immunostain demonstrated that Nox4 protein was increased in glomerular cells of Ad–/– kidneys and reduced with gAd treatment. Photomicrographs are representative of 50 glomeruli from each mouse kidney (n = 4 per group). (F) Quantitation of Nox4-positive cells per glomerulus (n = 4 per group). *P < 0.05 versus WT; **P < 0.05 versus Ad–/–. Values are mean ± SEM. Original magnification, ×40 (B–E); ×10 (D, insets).