Figure 3. HIV/SIV-related hematopoietic failure is dependent on a central Pro-rich motif of Nef.

(A) CD34⁺ cells from control animals were incubated for 48 hours with rNef (0.15 μM) or the indicated pools of Nef peptides (100 nM). (B) Alternatively, CD34⁺ cells were incubated with either wild-type or mutated (P₇₂, P₇₅, P₇₈) peptide B (100 nM). Cells were then processed for CFC assays.