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. 2008 Apr 22;118(5):1877–1889. doi: 10.1172/JCI34308

Figure 3. Pin1 has opposite effects on the protein stability of WT tau and P301L tau in brain-slice cultures.

Figure 3

Brain tissue slices of 230 μm were prepared and cultured from WT tau Tg mice in the presence (Pin1-WT) or absence (Pin1-KO) of Pin1 (A and B), from Tg P301L tau mice in the presence (Pin1-WT) or absence (Pin1-KO) of Pin1 (C and D), or from Thy1-Pin1 Tg mice (Pin1-Tg) or control non-Tg mice (Pin1-WT) (E and F). Cycloheximide was added to stop new protein synthesis and chased for indicated times. Brain slices were lysed in a buffer by sonication, followed by subjecting soluble protein lysates to immunoblotting with Tau5 or anti-actin antibodies (A, C, and E). Tau levels were semiquantified from 2 independent experiments using ImageQuant and normalized using actin as an internal control (B, D, and F).