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. 2008 Apr 22;118(5):1877–1889. doi: 10.1172/JCI34308

Figure 4. Pin1 KO in Tg P301L tau mice reduces tau hyperphosphorylation, NFT-specific conformations, and aggregation in the brain.

Figure 4

(A and B) Brains of 6- to 8-month-old non-Tg or Tg P301L tau mice in the presence or absence of Pin1 KO, as indicated, were homogenized, followed by subjecting total lysates (A) or sarkosyl-insoluble (Sar. insoluble) fractions (B) to immunoblotting with different tau mAbs with the following specificities: Tau5, specific against total human and mouse tau; CP27, specific against total human tau; AT180, CP9, and CP17, specific against pThr231-tau; AT8, specific against pSer202/pThr205-tau; AT100, specific against pThr212-tau in the NFT-specific conformation; MC1, specific against tau in the NFT conformation. (C) Subcellular localization of phosphorylated tau. Mice were perfused and fixed, and the coronal hippocampal sections were subjected to immunohistochemical staining using AT180. Similar results were also obtained with AT100 (not shown). Original magnification, ×20.