Figure 6. Pin1 overexpression in WT tau mice reduces tau hyperphosphorylation, NFT-specific conformations, and aggregation in the brain.

(A and B) Brains of 1-year-old non-Tg or Tg WT tau mice in the presence or absence of Pin1 overexpression, as indicated, were homogenized, followed by subjecting total lysates (A) or sarkosyl-insoluble fractions (B) to immunoblotting with different tau mAbs with the following specificities: Tau5, specific against total human and mouse tau; AT180, CP9, and CP17, specific against pThr231-tau; AT8, specific against pSer202/pThr205-tau; AT100, specific against pThr212-tau in the NFT-specific conformation; Alz50, specific against tau in the NFT conformation. (C) Subcellular localization of phosphorylated tau. Mice were perfused and fixed; this was followed by subjection of coronal spinal cord sections to immunohistochemical staining with AT180. Original magnification, ×20.