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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Exp Mol Pathol. 2007 Oct 17;84(1):1–8. doi: 10.1016/j.yexmp.2007.09.004

Fig. 2. Kinetics of IL-23p19 mRNA expression in microglia.

Fig. 2

Microglia were cultured with different doses of LPS and, at different time points, IL-23p19 mRNA expression was examined using real-time PCR. (A) Microglia were cultured with various concentrations of LPS (0, 0.01, 0.1, 0.5, and 1 µg/ml). After 24 h, p19 mRNA expression was determined by real-time PCR. Columns refer to mean values and bars to SEM of 3 independent experiments. (B) Microglia were cultured with 0.1 µg/ml LPS and harvested at different time points after culture (0, 4, 12, 24, 36, 48 hours). p19 mRNA expression was determined by real-time PCR. Symbols refer to mean values and bars to SEM of 3 independent experiments. (C) Microglia from 4 individual donors were cultured with 0.1 µg/ml LPS for 24 h. IL-23p19 mRNA expression was determined by real-time PCR. Un-stimulated microglia served as control. Columns refer to mean values and bars to SEM of 4 individual donors.