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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Exp Mol Pathol. 2007 Oct 17;84(1):1–8. doi: 10.1016/j.yexmp.2007.09.004

Fig. 4. The requirement of activated p38 MAPK for the regulation of IL-23 production.

Fig. 4

Microglia were cultured with indicated concentrations of LPS for 20 min and lysed for western blot. Activation of p38 MAPK was determined by anti-phospho-p38 mAb. Serial diluted SB203580 was pre-incubated 1 h after LPS addition. IL-23 concentration was assayed by ELISA. (A) LPS elicits rapid activation of p38 demonstrated by its phosphorylation in western blot. (B) Presence of SB203580 inhibits activation of p38. (C) The reduction of IL-23 in the microglia incubated with varied concentrations of SB203580. The viability of cells was 95–97% as determined by trypan blue. *, p<0.05, compared with the group of LPS+SB203580− microglia. One representative of two is shown.