Figure 2.
Sulfoamino acid analysis of protein synthesized by Tpst DKO tissues. Tissue explants were metabolically labeled with Na35SO4 and sulfoamino acid analysis was performed as described in Materials and Methods. A. Analysis of secreted proteins (left) and total cellular protein (right) from fetal livers at E15.5. B. Analysis of secreted proteins (left) and total cellular protein (right) from skin at P20. [35S]sulfotyrosine from wild type (lanes 1) and Tpst DKO (lanes 2) were visualized by autoradiography. Internal standards were visualized with ninhydrin (S). The origin is indicated by an asterisk. PR = phenol red.