Table 1.
PCR microarray and qRT-PCR values across the CENP-A major and minor domains
| PCR fragment namea | Distance from fragment 1b | Size (bp) | qRT-PCR value (Figure 3) | CENP-A ChIP cut-off ≤ 0.68, positive ≥ 1.02c | CENP-C ChIP cut-off ≤ 0.31, positive ≥ 0.65c | CENP-H ChIP cut-off ≤ 0.56, positive ≥ 0.63c | UCSC Human Genome Browser (hg17) genome coordinates |
| 1 | 0 | 569 | 0.72(-/+) | 1.23(+) | 0.59(-/+) | 101,899,991-101,900,560 | |
| (1) | 316 | 247 | 1.01(+) | 101,900,060-101,900,307 | |||
| 2 | 2,064 | 566 | 0.51(-) | 1.03(+) | 1.03(+) | 101,901,489-101,902,055 | |
| (2) | 1,965 | 150 | 1.15(+) | 101,901,806-101,901,956 | |||
| C | 6,166 | 178 | 1.78(+) | 101,905,979-101,906,157 | |||
| 3 | 7,462 | 796 | 0.60(-) | 2.28(+) | 1.17(+) | 101,906,657-101,907,453 | |
| (3) | 7,184 | 213 | 2.22(+) | 101,906,962-101,907,175 | |||
| 4 | 8,225 | 706 | 1.34(+) | 1.70(+) | 1.62(+) | 101,907,510-101,908,216 | |
| D | 12,840 | 223 | 1.80(+) | 101,912,608-101,912,831 | |||
| 10 | 15,300 | 423 | 0.31(-) | 0.40(-) | 0.15(-) | 101,914,868-101,915,291 | |
| 11 | 16,222 | 649 | 0.71(-/+) | 0.67(+) | 0.72(+) | 101,915,564-101,916,213 | |
| (11) | 15,830 | 247 | 2.76(+) | 101,915,574-101,915,821 | |||
| 26 | 53,983 | 861 | 1.17(+) | 0.83(+) | 0.64(+) | 101,953,974-101,954,835 | |
| 27 | 55,119 | 610 | 1.21(+) | 1.74(+) | 1.53(+) | 101,954,501-101,955,110 | |
| (27) | 54,554 | 190 | 3.44(+) | 101,954,355-101,954,545 | |||
| 35 | 80,686 | 649 | -1.21(-) | 0.07(-) | -0.25(-) | 101,980,028-101,980,677 | |
| (35-1) | 80,226 | 172 | 0.86(-) | 101,980,045-101,980,217 | |||
| (35-2) | 80,432 | 203 | 0.73(-) | 101,980,220-101,980,423 | |||
| 36 | 81,822 | 450 | 1.72(+) | 1.88(+) | 1.82(+) | 101,981,363-101,981,813 | |
| 37 | 82,493 | 350 | 1.17(+) | 1.56(+) | 1.56(+) | 101,982,134-101,982,484 | |
| 38 | 85,610 | 786 | -0.03(-) | 0.41(-/+) | 0.50(-) | 101,984,815-101,985,601 | |
| (38) | 85,501 | 143 | 0.48(-) | 101,985,349-101,985,492 | |||
| 39 | 87,099 | 924 | 1.62(+) | 0.50(-/+) | 0.93(+) | 101,986,166-101,987,090 | |
| 40 | 87,859 | 779 | 1.49(+) | 0.76(+) | 1.19(+) | 101,987,071-101,987,850 | |
| 90 | 246,368 | 430 | -0.06(-) | 0.29(-) | 0.04(-) | 102,145,930-102,146,359 | |
| (90) | 246,170 | 231 | 1.12(+) | 102,145,930-102,146,161 | |||
| 91 | 250,586 | 597 | 1.21(+) | 0.80(+) | 0.82(+) | 102,149,980-102,150,577 | |
| (91) | 250,432 | 187 | 1.43(+) | 102,150,236-102,150,423 | |||
| 92 | 251,863 | 534 | 1.33(+) | 1.07(+) | 1.14(+) | 102,151,320-102,151,854 | |
| (92) | 251,830 | 236 | 1.34(+) | 102,151,585-102,151,821 | |||
| S | 253,879 | 158 | 2.65(+) | 102,153,712-102,153,870 | |||
| T | 255,702 | 189 | 4.22(+) | 102,155,504-102,155,693 | |||
| 93 | 258,459 | 481 | 1.14(+) | 0.65(+) | 0.37(-) | 102,157,969-102,158,450 | |
| (93) | 258,331 | 245 | 2.30(+) | 102,158,077-102,158,322 | |||
| U | 259,157 | 224 | 0.99(+) | 102,158,924-102,159,148 | |||
| V | 265,411 | 169 | 0.26(-) | 102,165,233-102,165,402 |
aNumbers indicate polymerase chain reaction (PCR) microarray fragments as in Figure 2; numbers in parentheses indicate quantitative real-time (qRT)-PCR fragments wholly contained within the PCR microarray fragment with same number (Figure 3); letters indicate qRT-PCR fragments only (Figure 3). Different regions within the major domain and the minor domain are indicated by alternating bold and regular rows. bDistances are taken from the first coordinate in fragment 1 to the last coordinate of the next fragment. cValue given in Figure 2 for PCR microarray chromatin immunoprecipitation (ChIP): (+), ≥ threshold for positive values; (±), weakly positive, over cut-off but below the threshold for positive values; (-), below the cut-off value. CENP, centromere protein.