Figure 1. Ngb protein expression and cell viability in PC12 cells after transfection of Ngb siRNA and treatment of H₂O₂.

PC12 cells were transfected with Ng siRNA or control siRNA for 48 h. Following transfection, the cells were treated with H₂O₂ (0.1mM) or same volume of H₂O (control) for 6 h. Ngb protein expression was determined by quantitative western blotting. Cell viability was assessed by MTT assay. 1A, Ngb protein expression following transfection of Ngb siRNA and treatment of H₂O₂. Data were expressed as a percentage of control siRNA in control group (n=6/group, *P<0.01 vs. control, #P<0.01 vs. control siRNA). 1B. Cell viability following transfection of Ngb siRNA and treatment of H₂O₂. Data were expressed as absolute OD values (570 nm OD) (n=6/group, *P<0.01 vs. control, #P<0.01 vs. control siRNA).