Figure 2.

Analysis of the effects on mating-type switching of deleting abp1 in h⁰⁹ cells. (A) Schematic representation of the structural organisation of the mating-type region in an h⁰⁹ strain. The positions of mat1, mat2M and mat3P loci are indicated. The position of the SSB/DSB imprint is also indicated. (B) Iodine staining of wild-type (h⁰⁹) and abp1Δ colonies. Numbers below each panel correspond to the frequencies of sporulation. (C) Quantitative multiplex PCR determination of the predominant mating type adopted by wild-type h⁰⁹ (lanes 1–3) and abp1Δ cells (lanes 4–6). PCR reactions were performed as in Figure 1D. The M/P ratios (±s.d.) are indicated below the corresponding lanes.