Figure 3.

Abp1 localises to the silent domain of the mating-type locus. (A) Binding of Abp1–HA at the silent domain of the mating-type locus was determined by ChIP-analysis using αHA antibodies. Immunoprecipitated material was analysed by multiplex PCR using primers specific for the indicated regions of the mating-type locus (bands ir, r2, c1, c2, l1 and l2) (see Supplementary Table SII for a description of the primers used) and, in the same PCR reaction, primers that amplify a fragment of similar length of the act1 locus (bands act), used as control. (B) Similar experiments as those described in (A) but with primers designed to amplify shorter DNA fragments flanking region l1 (bands p1, p2 and p4) or contained within it (bands p3). In this case, a shorter fragment from the act1 gene was used as control (bands act(s)). Lanes WCE correspond to PCR products obtained from the input material before immunoprecipitation. Lanes αHA correspond to the products obtained from the immunoprecipitated material. Numbers below each lane correspond to the ratio of the corresponding mating-type-specific band with respect to the control act1 band.