Figure 4.

Analysis of the effects on mating-type switching of deleting cbh1 and cbh2 in h⁹⁰ cells. (A) Quantitative multiplex PCR determination of the predominant mating type adopted by cbh2Δ (lanes 1–3), cbh1Δ (lanes 4–6) and cbh1Δcbh2Δ cells (lanes 7–9). (B) Quantitative multiplex PCR determination of the predominant mating type adopted by abp1Δcbh2Δ (lanes 1–3) and abp1Δcbh1Δ cells (lanes 4–6). PCR reactions were performed as in Figure 1D. The M/P ratios (±s.d.) are indicated below the corresponding lanes.