Table 2.
List of strains and plasmids used in this study
| Strain/plasmid | Relevant genotype | Reference and/or source |
|---|---|---|
| Strains | ||
| BL21(λDE3) | HsdS gal ompT rB− mB− [ΔC1857(Ts)indI Sam7 min5 lacUV5-T7 gene 1] | Studier et al (1990), Novagen |
| BL21(λDE3)pLysS | HsdS gal ompT rB− mB− [ΔC1857(Ts)indI Sam7 min5 lacUV5-T7 gene 1] | Studier et al (1990), Novagen |
| RP437 | Wild type for chemotaxis; his thr leu metE thi eda rpsL | Parkinson (1978) |
| DW35 | zjd∷Tn10Δ(frdABCD)18sdhC∷kan | Westenberg et al (1993) |
| RP437Δsdh | Wild type for chemotaxis; his thr leu metE thi eda rpsL; Δsdh | Montrone (1996) |
| RP437Δfrd | Wild type for chemotaxis; his thr leu metE thi eda rpsL; Δfrd | This study |
| RP437ΔsdhΔfrd | Wild type for chemotaxis; his thr leu metE thi eda rpsL; ΔsdhΔfrd | This study |
| DFB225ΔfliG | RP437 parent containing an fliG in-frame deletion | Received from David Blair (Lloyd et al, 1996) |
| M15 [pREP4] | LacZ StrRKanR | Zamenhof and Villarejo (1972), QIAGEN |
| Plasmids | ||
| pQE12-CheY-His tag | 6 × His–CheY overproducing | Received from A Wolfe |
| pKOT107 | FliG and FliF overproducing | Oosawa et al (1994) |
| pFAS | SDH overproducing | Maklashina et al (1998) |
| pKLR2 | FliM and FliN overproducing | Oosawa et al (1994) |
| pEWG1 | 10 × His–FliG overproducing | This study |
| pEWG2 | 10 × His–FliG | This study |
| pBAD24 | Expression vector containing the PBad promoter; Apr | Guzman et al (1995) |
| pH3 | FRD overproducing | Blaut et al (1989) |
| pEWF1 | FRD single-copy producing | This study |