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. 2008 Mar 6;27(7):934–947. doi: 10.1038/emboj.2008.40

Figure 5.

Figure 5

Depletion of ZFPL1 alters the steady-state distribution of GM130. (A) HeLa cells were transfected with control (luciferase) siRNA or siRNA targeting ZFPL1, incubated for 72 h and analysed by western blotting with antibodies to the indicated proteins. The asterisk indicates a protein that cross-reacts with the anti-ZFPL1 antibody by western blotting. (B) SiRNA-treated cells were fixed 72 h post-transfection, double-labelled with antibodies to ZFPL1 (red) and GM130 (green) and visualized by epifluorescence microscopy. DNA is blue. (C) SiRNA-treated cells were analysed 72 h post-transfection by confocal microscopy. Cells were double-labelled with antibodies to GM130 (green) and either GalNacT2 (red, top panel), ERGIC53 (red, middle panel) or p115 (red, bottom panel). Scale bar, 10 μm.