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. 2008 Mar 6;27(7):934–947. doi: 10.1038/emboj.2008.40

Figure 6.

Figure 6

GM130 tubules are exaggerated upon ZFPL1 depletion. (A) HeLa cells were transfected with control (luciferase) siRNA or siRNA targeting ZFPL1 as indicated, incubated at 37°C for 72 h, then shifted to 15°C for 3 h before fixation and double labelling with antibodies to GM130 (green) and GalNacT2, ERGIC53 or p115 (red). Cells were visualized by confocal microscopy. (B) SiRNA-treated cells were incubated for 48 h at 37°C, then transiently transfected with myc-tagged GM130 and incubated for a further 24 before fixation and double labelling with antibodies to myc (green) and GalNacT2, ERGIC53 or p115 (red). Cells were visualized by confocal microscopy. Scale bar, 10 μm.