Figure 7.

GM130 punctae and tubules formed upon ZFPL1 depletion contain anterograde cargo. HeLa cells were transfected with control (luciferase) siRNA or siRNA targeting ZFPL1, incubated for 48 h, then infected with adenovirus encoding ts-O45 VSV-G–GFP (green) and incubated at 40°C overnight. Cells were shifted to 15°C for 3 h and either fixed directly (top) or transferred to 31°C for a further 10 min before fixation (bottom). Cells were labelled with anti-GM130 antibodies (red) and visualized by epifluorescence microscopy. Scale bar, 10 μm.