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. 2008 Mar 13;27(7):1110–1121. doi: 10.1038/emboj.2008.31

Figure 2.

Figure 2

TLR7 ligands induce autophagy. (A) Quantification of GFP–LC3 puncta (⩾1 μm) in RAW 264.7 macrophages cells co-transfected with GFP–LC3 and either control scrambled siRNA (sc) or Beclin 1 siRNA. After 22 h cells were incubated for 4 h with complete media alone (C) or in the presence of 10 μg/ml imiquimod (Imiq) or 10 μg/ml ssRNA, or incubated for the last 2 h in starvation medium (Starv). Data are means±s.e.m. (n=6); **P<0.01 (ANOVA). (B) RAW 264.7 macrophages were transfected with control scrambled siRNA (sc) or Beclin 1 siRNA and after 24 h cells were lysed and analysed by western blotting using anti-Beclin 1 or anti-GAPDH antibodies. (C) Primary BMMs derived from GFP–LC3 transgenic mice were incubated for 2 h with starvation media or 4 h with complete media alone (control) or in the presence of 10 μg/ml imiquimod or 10 μg/ml ssRNA. Bar, 5 μm. (D) Quantification of GFP–LC3 puncta (⩾1 μm) in BMMs expressing GFP–LC3 in panel C. Cells were stimulated with complete media alone (C), imiquimod (Imiq) or ssRNA. Data are means±s.e.m. (n=3). Exact P-values (ANOVA) are shown. A full-colour version of this figure is available at The EMBO Journal Online.