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. 2008 Mar 20;27(7):1097–1109. doi: 10.1038/emboj.2008.41

Figure 2.

Figure 2

piggyBac DSBs occur by means of a hairpin intermediate on the transposon end. (A) DNA hairpin formation is visualized using a pre-nicked end. A pre-nicked piggyBac L-TIR was incubated with piggyBac transposase in the presence of a target DNA for various times and then displayed on a denaturing acrylamide gel. (B) Hairpin formation requires a 3′OH transposon end. A pre-nicked piggyBac L-TIR with 3′G-OH (deoxy) or 3′G-H (dideoxy) was incubated with piggyBac transposase in the presence of a target DNA for 5 or 20 min and then displayed on a denaturing acrylamide gel. Lanes 1–3, piggyBac L-TIR with 3′G-OH (deoxy); lanes 3–5, piggyBac L-TIR with 3′G-H (dideoxy); lanes 1 and 3, ‘no protein' controls.