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. 2008 Mar 20;27(7):1097–1109. doi: 10.1038/emboj.2008.41

Figure 6.

Figure 6

The flanking donor sequence influences transposon end processing. (A) Flanking sequence and 3′OH end nicking. piggyBac transposase was incubated with L-TIR fragments flanked by donor DNA of different sequences, as indicated, for 20 min and reactions displayed on a denaturing acrylamide gel. (B) Flanking donor DNA influences hairpin formation. Pre-nicked piggyBac L-TIRs with either a TTAA or GCGC flank were incubated with piggyBac transposase in the presence of a target plasmid and displayed on a denaturing gel. (C) Influence of flanking sequence on target joining. The reactions using the pre-nicked substrates were displayed on a native gel.