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. 2008 Mar 6;27(7):1049–1059. doi: 10.1038/emboj.2008.42

Figure 6.

Figure 6

ER stress induces HSR in vivo. (A) IRE1 (MATa W303) and ire1Δ (KKY100) cells bearing an HSE-LacZ reporter (pCM64-SSA3-lacZ) and vector (pRS315) or pGAL-CPY* (pES67) were grown in SC medium with 2% raffinose at 30°C. CPY* was then induced in 2% galactose medium for 2 h. β-Galactosidase activity was measured in cell lysates. The graph shows the mean±s.e. of three independent experiments. (B) IRE1 (MATa W303) and ire1Δ (KKY100) cells transformed with an HSE-LacZ reporter (pCM64-SSA3-lacZ) were treated with tunicamycin (5 μg/ml) at the indicated times. β-Galactosidase activity was measured in cell lysates; activity from cells incubated in DMSO was subtracted. The graph shows the mean±s.e. of three independent experiments.