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. 2008 Jan 25;4(1):e14. doi: 10.1371/journal.ppat.0040014

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© 2008 Watson and Galán.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Human intestinal epithelial T84 cells were infected with C. jejuni and viable intracellular bacteria were assessed over time by CFU recovery (A and B) or live/dead staining (B–D).

(A) Viable CFU of intracellular C. jejuni were determined at the indicated times after gentamicin treatment. (B) Viable intracellular C. jejuni was analyzed at the indicated times via CFU recovery as in A or live/dead staining and FACS analysis as described in the Materials and Methods. (C) Epiflourescent visualization of a representative field of intracellular C. jejuni isolated at 4 h and 24 h post-infection and subjected to live (green)/dead (red) staining. (D) Intracellular C. jejuni was isolated at different times after infection and the number of viable and non-viable intracellular bacteria was quantified via live/dead staining and FACS analysis. Values are expressed as percent of live bacteria. In all cases, values are the mean and standard deviation of three independent experiments.