Figure 2. C. jejuni Remains Viable within Intestinal Epithelial Cells but Does Not Survive within Bone Marrow–Derived Macrophages.

(A) Human intestinal epithelial T84 cells were infected with C. jejuni, and viable intracellular bacteria were assessed over time by CFU recovery under 10% CO₂ or oxygen-limiting conditions as described in Materials and Methods. (B) The number of viable intracellular bacteria was analyzed in parallel via CFU recovery plated under oxygen-limiting conditions or live/dead staining coupled with FACS analysis. (C) Mouse bone marrow–derived macrophages were infected with C. jejuni and viable bacteria CFU were determined over time after 10% CO₂ or oxygen-limiting cultivation. All values are the mean and standard deviation of three independent determinations.